发明名称 |
TEMPERATURE SENSITIVE POLYMERASE INHIBITOR, THERMOSTABLE DNA POLYMERASE COMPOSITION CONTAINING SAID INHIBITOR AND METHOD FOR AMPLIFICATION OF TARGET NUCLEIC ACID |
摘要 |
Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T1 which is generally below 85<o>C. The inhibitors are irreversibly inactivated at temperature T2 which is generally above 40<o>C. T2 is also greater than T1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR. <IMAGE> |
申请公布号 |
JPH06209775(A) |
申请公布日期 |
1994.08.02 |
申请号 |
JP19930274812 |
申请日期 |
1993.10.07 |
申请人 |
EASTMAN KODAK CO |
发明人 |
EDOWAADO ROBAATO SUKARAISU;DEBITSUDO JIYON SHIYAAKII;KENESU JIYOOJI KURISUTEI JIYUNIA;SEODOA UORUTAA ESUDAASU;JIYON RINFUOOSU DAISU |
分类号 |
C12N9/98;C07K16/40;C12N9/12;C12N15/09;C12P21/08;C12Q1/68;C12R1/91;G01N33/53;(IPC1-7):C12N9/98;C12N15/10;C12N15/06 |
主分类号 |
C12N9/98 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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