| 摘要 |
PURPOSE:To inexpensively obtain a VA mycorrhizal fungus useful for preparation of VA mycorrhizal fungus having high spore density and excellent activity by making a VA mycorrhizal fungus symbiosing in a root of a plant, applying a compound having cytokinin action, culturing the plant infected with the VA mycorrhizal fungus. CONSTITUTION:Red granular soil sterilized with methyl bromide is packed into a flowerpot, 80 spores of VA mycorrhizal fungus [e.g. Gigaspora margarita (trust is rejected by Fermentation Research Institute) are inoculated into the soil, seeds of a plant such as dent corn are sowed, the red granular soil is packed on the seeds and the plant is cultured in a greenhouse at 25-30 deg.C. A VA mycorrhizal fungus is made to symbiose in the root of the plant, a compound (e.g. transzeatin) having action as cytokinin is applied to the plant infected with the VA mycorrhizal fungus and the plant infected with the VA mycorrhizal fungus is culture to inexpensively multiply the objective VA mycorrhizal fungus useful for preparation of the VA mycorrhizal fungus having high spore density and excellent activity. |
