| 摘要 |
PURPOSE: To obtain a nutrient that is rich in vitamin E and useful for healthy foods and the like without producing a waste, by culturing spores of Lentinum edodes in a slop medium, further culturing the cultured product in a liquid medium, homogenizing the fermented liquid to mix other components and spray-drying the liquid. CONSTITUTION: Spores of Lentinum edodes are inoculated in a slop agar medium to culture at a temp. of 20 to 30 deg.C for 10 to 18 days. The cultured product is inoculated in a liquid medium prepared from natural foods and components for foods including a starch as a carbon source and defatted soy beans, defatted peanut, or plumule of wheat as a nitrogen source, and cultured at a temp. a 20 to 30 deg.C for 2 to 8 days while stirring in a rather slow stirring rate as 100 to 200 rpm in the initial stage and then in a higher rate as 200 to 300 rpm in the final stage. The mycelium of the fermented liquid is broken and the fermented liquid is treated in a high pressure homogenizer. Then saccharose, glucose, aspartame, citric acid, caboxymethylcellulose, etc., are added to the fermented liquid, and if necessary, the liquid is concentrated under reduced pressure, and then spray dried at an entrans temp. of 160 to 200 deg.C and at an exit temp. of 100 to 120 deg.C to obtain the SHIITAKE (a Japanese mushroom) nutrient of the purpose. |
