DIAGNOSTIC ASSAYS AND KITS FOR RNA USING RNA BINARY PROBES AND AN RNA-DIRECTED RNA LIGASE

摘要

There are provided nucleic acid hybridization assays for RNA targets using RNA binary probes and a protein that is an RNA-directed RNA ligase. Preferred assays include generation of an exponentially amplified signal. Most preferred assays include T4 DNA ligase as the RNA-directed RNA ligase. Also provided are kits for performing assays according to this invention.