摘要 |
A method for screening or detection of a non-enzyme catalytic polypeptide or protein for the conversion of a substrate S to a product P is provided, in which a preparation containing the potential catalyst is contacted with the substrate S immobilized to a support and the immobilized product P obtained is detected, preferably by immunoassay. The method is preferably used for the screening of hybridoma supernatants for catalytic monoclonal antibodies that catalyze acyl transfer reactions, e.g., hydrolysis or aminolysis, condensation reactions and resolution of enantiomers. |
申请人 |
YEDA RESEARCH AND DEVELOPMENT CO. LTD.;YISSUM RESEARCH AND DEVELOPMENT COMPANY OF THE HEB;ESHHAR, ZELIG;GREEN, BERNARD, S.;TAWFIK, DAN, S. |
发明人 |
ESHHAR, ZELIG;GREEN, BERNARD, S.;TAWFIK, DAN, S. |