METHOD OF USING PRESERVED CONTROL CELLS IN THE CALIBRATION OF FLUORESCENT AND LIGHT SCATTER MEASUREMENTS

摘要

The invention describes a method of using differently labelled, reconstituted preserved cells as control cells in multiple color assays. These cells are used to calibrate light scatter and fluorescent intensity measurements. The use of said control cells overcomes the preservation and calibration difficulties experienced in the conventional use of fresh cells and polymer beads as controls in determining the compensation adjustments required in multiple color flow cytometry assays as a result of the overlapping wavelength regions encountered.