AFFINITY PURIFICATION BY THE USE OF A COMPLEXED LIGAND

摘要

Method for affinity based isolation of a first target substance, said method comprising: (i) contacting said first target substance with a matrix to which is linked a ligand having affinity for said first target substance; (ii) eluting said first target substance. The method is characterized by the ligand having been initially complexed/bound to a second target substance having weaker binding ability to the ligand than the first target substance and providing multi-point attachment to the matrix by carrying several ligand binding sites and/or other matrix binding sites on its molecule enabling adsorption of the first target substance during step (i) without the second target substance becoming eluted from the matrix. In one mode the second target substance is thermo-reactive, and steps (i) and (ii) are run at different temperatures separated by the cloud point of the second target substance.