| 摘要 |
PCT No. PCT/SE89/00144 Sec. 371 Date Nov. 26, 1990 Sec. 102(e) Date Nov. 26, 1990 PCT Filed Mar. 17, 1989 PCT Pub. No. WO89/09265 PCT Pub. Date Oct. 5, 1989.This invention relates to an apparatus for automatic purification of extra-chromosomal DNA from a cell suspension in a container. The apparatus comprises a device for introducing the cell suspension from a container into a chamber; a device for agitating the contents of the chamber; a device for introducing a lysing solution to the chamber to lyse the cells therein; a device for introducing a precipitating solution into the chamber to precipitate chromosomal DNA and, optionally, proteins and cellular debris therein; a device for feeding the liquid contents of the chamber through a filter to a collecting system; a device for purifying extra-chromosomal DNA from the contents of the collecting device; a device for introducing a dissolving solution into the chamber to dissolve the precipitate remaining therein, and a device for feeding the dissolved precipitate to waste.
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