ENZYME-CATALYZED CHEMILUMINESCENT DETECTION OF PROTEINS AND NUCLEIC ACIDS USING HYDROXYARYL CYCLIC DIACYLHYDRAZIDE COMPOUNDS

摘要

A method and test kit using a hydroxyaryl cyclic diacylhydrazide, a peroxide and a peroxidase enzyme on a blotting membrane for detecting DNA, RNA or proteins (polypeptides) is described. The method and kit provide enhanced chemiluminescence in an assay because of the use of the membrane. The method and test kit is particularly useful in Western, Southern and Northern blotting type assays and DNA sequencing.