IMPROVED YIELDING OF PCR PRODUCTS IN THE PRESENCE OF SINGLE-STRAND DNA BINDING PROTEIN AND PREPARATION THEREOF FROM THERMUS FLAVUS

摘要

The execution of polymerase chain reactions is improved by adding to the reaction mixture preparations of proteins from a thermophilic organism which are capable of binding single strand DNA. Preparations which can be used may be produced from thermophilic organisms, in particular Thermus flavus, strain At-62 (DSM No. 674), by fractionating a raw extract obtained from cells of such an organism.