DNA ENCODING THE SPH I RESTRICTION ENDONUCLEASE AND METHODS FOR PRODUCING THE SAME

摘要

The present invention is directed to a method for cloning and producing the SphI restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces phaeochromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity, and 3) purifying the SphI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity.