Fluorescent stain containing pyrylium salt or its similar salt, detection method of nucleic acid by use of it, and fluorescent staining method of biological sample.

摘要

A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R<1>, R<2> and R<3>, wherein each of R<1> and R<2> is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R<3> is -A or -L-A, L is -L<1>-, -L<2>-L<3>- or -L<4>-L<5>-L<6>-, and each of L<1> to L<6> is independently -(CH=CH)-, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y<-> is an anion. A method for detecting a nucleic acid which comprises the steps of reacting a sample with the stain, and then detecting, by an optical means, the double-strand nucleic acid stained with the stain in the case that the double-strand nucleic acid is contained in the sample.