发明名称 Method and apparatus for automatic measurement of fluorescence
摘要 An analyzer has a reaction disk for holding a plurality of reaction containers and a fluorophotometer for measuring fluorescence stemming from solutions in the containers. Most of the containers contain solid phases attached with antibodies but at least one container does not contain any solid phase. In normal operation of the analyzer, a test sample containing antigens and a latently fluorescent reagent such as an antibody labeled by an enzyme are added to a container containing a solid phase. In this container, a fluorescent substance is created through an enzyme reaction. Light is irradiated on the container and the fluorescence emitted from the fluorescent substance is measured. While measuring test samples, fluorescence stemming from a reference sample, such as quinine sulfate, is measured to produce values for the reference sample by which measured values for the test samples are corrected. Prior to measuring general test samples by the analyzer, fluorescence stemming from a reference sample in a container removed of any solid phase is measured and compared with a setting value. If the results of the comparison indicate that the normal operation will be difficult to continue, then the sampling operation by the pipetter is discontinued.
申请公布号 US5324635(A) 申请公布日期 1994.06.28
申请号 US19890397853 申请日期 1989.08.24
申请人 HITCAHI, LTD. 发明人 KAWASE, MASUMI;IMAI, KYOKO;HASHIMOTO, HIROSHI
分类号 G01N33/536;G01N21/64;G01N33/543;G01N33/76;G01N33/78;G01N35/00;G01N35/02;G01N35/04;(IPC1-7):G01N33/535 主分类号 G01N33/536
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