A foreign substance, such as DNA, is injected into plant cells or tissue by a biological ballistical insertion technique. High pressure propellant gas is employed to accelerate the macroprojectile containing microprojectiles coated with the foreign substance. A movable macroprojectile stop mechanism is provided between an upper injection chamber and a lower sample-holding chamber so that multiple shots of macroprojectiles can be made without disrupting the vacuum in the lower chamber. The stop mechanism includes removable stopper plugs for stopping the macroprojectile. A vacuum seal is provided between the stop mechanism and the lower chamber.
申请公布号
DE69006516(T2)
申请公布日期
1994.06.23
申请号
DE1990606516T
申请日期
1990.12.17
申请人
THE UPJOHN CO., KALAMAZOO, MICH.
发明人
JONES, LAWRENCE, DUANE, PORTAGE, MI 49002;FREY, PAUL, TIMOTHY, DELTON, MI 49046;GLEASON, DAVID, DUANE, KALAMAZOO, MI 49009;CHEE, PAUL, P., KALAMAZOO, MI 49008;SLIGHTOM, JERRY, L., KALAMAZOO, MI 49001