THE USE OF THE KLUYVEROMYCES MARXIANUS INULINASE GENE PROMOTER FOR PROTEIN PRODUCTION

摘要

The invention provides a nucleic acid sequence derivable from a yeast, e.g. from a Kluyveromyces yeast, preferably from the strain K. marxianus var. marxianus (CBS 6556), and comprising at least a regulatory region derivable from a gene encoding a polypeptide having inulinase activity or a functional modification thereof. Regulatory regions comprise a promoter, a terminator, and a sequence encoding a secretory signal necessary for secreting a gene product from a yeast. The DNA sequence of the inulinase gene from K. marxianus var. marxianus (CBS 6556) is given, including its regulatory regions. The regulatory regions can be used for preparing vectors suitable for transforming a host, preferably a yeast, for the production of desired expression products, e.g. proteins, RNA suitable for flavouring purposes, and metabolites. A process for production of an expression product is also provided.