发明名称 |
Purified coriolus versicolor polypeptide complex |
摘要 |
A method of obtaining a novel polypeptide from a crude extraction product of polysaccharide peptide Coriolus versicolor comprising: a) boiling a water soluble powder of polysaccharide peptide Coriolus versicolor; b) centrifuging a boiled product from step a); c) filtering a centrifuged product from step b); d) purifying a solution from step c) by gel filtration chromatography; e) subjecting the purified material from step d) to HPLC using a reversed-phase at ambient temperature, f) subjecting the purified material from step e) to capillary isoelectrophoresis focusing; g) further purifying this product by HPLC and ionic exchange columns and h) purifying a protein by SDS-PAGE and i) recovering a peptide from 12 Kd to 16 kD. The peptide has the partial amino acid sequence GTAAAKEFERQHM SEQ ID NO:1. |
申请公布号 |
AU3230393(A) |
申请公布日期 |
1994.06.22 |
申请号 |
AU19930032303 |
申请日期 |
1992.11.30 |
申请人 |
DEPARTMENT OF THE ARMY |
发明人 |
MABLE MEI PO YANG;GEORGE CHEN |
分类号 |
A61K36/07;A61K38/00;C07K1/14;C07K17/00 |
主分类号 |
A61K36/07 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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