| 摘要 |
Oligodeoxynucleotides are rendered more resistant to degradation by nucleases by the inclusion of a base sequence at the 3' end which forms a hairpin loop-type secondary structure. The hairpin loop sequence forms an extremely stable structure comprised of a double stranded stem and loop region, 3' to the single stranded antisense sequence. The hairpin loop structures are used advantageously in stabilising antisense oligodeoxynucleotides which are used to block gene expression DOLLAR (in vivo) or DOLLAR (in vitro). |
