发明名称 |
The manufacture and expression of large structural genes. |
摘要 |
<p>There is provided a method for the manufacture of linear, double stranded DNA sequences of a length in excess of about 200 base pairs and coding for expression of a predetermined continuous sequence of amino acids with a selected host microorganism transformed by a selected DNA vector including the sequence, a manufactured, linear, double stranded DNA sequence of a length in excess of about 200 base pairs and coding for the expression of a predetermined continuous sequence of amino acids by a selected host microorganism transformed with a selected DNA vector including the sequence, said sequence characterized by having at least one unique palindromic six base recognition site for restriction endonuclease cleavage at an intermediate position therein, a polypeptide product of the expression by an organism of such a manufactured DNA sequence, a biologically functional DNA microorganism transformation vector including such a manufactured DNA sequence and a reagent material comprising a radio labelled manufactured DNA sequence.</p> |
申请公布号 |
EP0424990(B1) |
申请公布日期 |
1994.06.22 |
申请号 |
EP19900124234 |
申请日期 |
1983.04.25 |
申请人 |
AMGEN INC. |
发明人 |
ALTON, NORMAN K.;PETERS, MARY A.;STABINSKY, YITZHAK;SNITMAN, DAVID L. |
分类号 |
C12N15/09;A61K38/21;A61P43/00;C07H1/00;C07H21/04;C07K14/00;C07K14/52;C07K14/555;C07K14/56;C07K14/565;C07K14/57;C07K16/00;C12N;C12N1/00;C12N1/21;C12N15/00;C12N15/10;C12N15/20;C12N15/21;C12N15/23;C12N15/70;C12N15/71;C12P19/34;C12P21/00;C12P21/02;C12Q1/68;C12R1/19;G01N33/532;G01N33/534;(IPC1-7):C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|