| 摘要 |
PURPOSE:To provide a new peptide having a specific amino acid sequence, exhibiting neurotrophic factor-like activity, having cholinergic neuron choliacetyl group transferase action and useful as an agent for the treatment of senile dementia, Alzheimer's dementia, etc. CONSTITUTION:Secretion exuded from the damaged part of the frontal corvex of a rat of 3 days old is collected, homogenized together with a tissue culture medium, centrifuged to collect the supernatant and subjected to 30-60% saturation ammonium sulfate fractionation. The active fraction is dissolved in a sodium phosphate buffer solution and filtered by get-filtration. The obtained active fraction is passed through an ion exchange resin column equilibrated with an acetate buffer solution (pH 5.0). The non-adsorbed fraction is collected and subjected to a stab gel electrophoresis with sodium dodecylsulfate(SDS)- polyacrylamide to collect a protein exhibiting single band and having a molecular weight of about 15,000 and a neurotrophic factor-like activity. The protein is decomposed with a protease and purified by the reversed phase high- performance liquid chromatography to obtain the subject new peptide having the amino acid sequence of formula. |
