PUTATIVE THERAPEUTIC TARGETS FOR THE PREVENTION AND STOPPAGE OF HUMAN ALZHEIMER'S DISEASE: CDNA AND AMINO ACID SEQUENCE OF A PROTEINASE/ESTERASE-LIKE PROTEIN AND A PROTEINASE/PROTAMINE, TRUNCATED, MODIFIED FORM OF THE PROTEIN, ANTI ENSE TO THE ALZHEIMER ELATED HUMAN APP GENE

摘要

cDNAs for a 104 amino acid protein with endoproteinase and acetylcholinesterase domains and for a cellular, modified, truncated version of the protein are constructed using oligonucleotide synthesis, and expressed in a prokaryotic vector. The sense-antisense relationship between the cDNAs encoding these proteins and the cDNA encoding the amyloid forming APP gene, and the similarities between the amino acid sequences deduced for the protein, and amino acid sequences in proteinases and esterases which are involved in pathogenic processes, make the proteins perfect candidates for factors that cause and maintain human Alzheimer's disease. Since the mRNAs encoding these proteins in healthy humans are repressed by mRNA encoding the normal APP gene and the gene encoding the amyloid related protein APRP363, they are potentially excellent targets for a safe, effective, immunological approach aimed at preventing, perhaps even stopping and reversing, the course of the disease.