发明名称 |
PREVENTING ENDOGENOUS AMINOPEPTIDASE MEDIATED N-TERMINAL AMINO ACID CLEAVAGE DURING EXPRESSION OF FOREIGN GENES IN BACTERIA |
摘要 |
A process for the recombinant preparation in a bacterial host of the mature form of a mammalian protein or peptide of formula X-Pro-Z, which is subject to processing by endogenous bacterial aminopeptidases is provided. In the formula X-Pro-Z, X is a single N-terminal amino acid other than proline, and Z is the remaining sequence of amino acid residues of the protein or peptide. The process comprises inserting into a cell of the bacterial host an appropriate vector containing DNA coding for Met-Y-X-Pro-Z, in which Y is a natural amino acid that is specifically cleavable in vitro from X-Pro-Z by aminopeptidase and that imparts resistance to in vivo processing by endogenous bacterial aminopeptidases. The cell is then induced to express the expression product Met-Y-X-Pro-Z which is then treated with an appropriate aminopeptidase to cleave off Met and Y.
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申请公布号 |
CA2145384(A1) |
申请公布日期 |
1994.06.09 |
申请号 |
CA19932145384 |
申请日期 |
1993.11.29 |
申请人 |
NOVARTIS AG;CIBA GEIGY AG |
发明人 |
HEITZMANN, MARKUS;MOVVA, RAO;RAMAGE, PAUL |
分类号 |
C12N15/09;C07K14/52;C07K14/54;C12N15/10;C12P21/02;C12P21/06;C12R1/19;(IPC1-7):C12N15/11;C12N15/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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