摘要 |
<p>Gel electrophoresis methods and compositions are provided, employing a fluorescent pH marker which allows for monitoring of the progress of the gel, and identifying the migration boundary and the pH at particular sites in the gel. By using pH gradients, particularly transverse gradients, pH-related migratory characteristic can be identified, so as to provide for a distinctive characteristic for a sample component and improve the opportunities to separate sample components which might migrate together at a particular pH.</p> |