| 摘要 |
<p>The present invention provides a process for amplifying a nucleic acid containing a DNA sequence of interest which comprises (a) lysing cells from a sample containing the nucleic acid; (b) mixing the lysed cells with about an equal volume of a buffer-saturated-phenol until an aqueous layer and an organic layer are formed and the nucleic acid is extracted into the aqueous layer; (c) removing the aqueous layer containing the nucleic acid; and (d) adding the aqeous layer so removed to a polymerase chain reaction mixture comprising deoxyribonucleotide triphosphates (dNTPs), sense and antisense primers, an amplification buffer, and between an amount of a DNA polymerase effective to catalyze polymerase chain reaction, under standard polymerase chain reaction conditions, thereby, thereby amplifying the DNA sequence of interest. The present invention also provides processes for converting RNA into cDNA and for amplifying a nucleic acid containing an RNA sequence of interest. Lastly, the present invention provides a process for determining a false positive signal from RT-PCR.</p> |
