| 摘要 |
<p>A method of obtaining a novel polypeptide from a crude extraction product of polysaccharide peptide Coriolus versicolor comprising: a) boiling a water soluble powder of polysaccharide peptide Coriolus versicolor; b) centrifuging a boiled product from step a); c) filtering a centrifuged product from step b); d) purifying a solution from step c) by gel filtration chromatography; e) subjecting the purified material from step d) to HPLC using a reversed-phase at ambient temperature, wherein a solvent composition is at an acidic pH and further includes KC1 solvent, and wherein an elution system consists of a linear gradient of about 80 % methanol applied at a rate of from between 0-40 minutes to obtain chromatographic peaks; f) analyzing said chromatographic peaks by monitoring for absorbance at about 230 nm, 1.0 AUFS for protein analysis and about 620 nm, 0.02 AUFS for polysaccharide analysis and collecting fractions of each peak in a microfractionator; g) filter-sterilizing each eluent of each chromatographic fraction peak, and drying product therefrom under reduced pressure; h) preparing a solution of an ampholyte mixture of said dried product and identifying structural components of said product by capillary isoelectrophoresis; and i) using gel filtration to obtain polypeptides products of from about 10K and to 50K.</p> |
