Method for cultivation of nitrile hydratase-producing pseudomonas

摘要

A method for the cultivation of bacteria of the genus Pseudomonas capable of producing nitrile hydratase is disclosed. The method involves adding a water soluble copper compound in an amount of about 0.5 to 5.0 mg/l as calculated in terms of copper to a culture medium in the preparation of cells of the bacteria under shear force supplied by stirring blades, wherein the circumferential speed of the edges of the stirring blades exceeds about 2.5 m/sec. The water soluble copper compound is preferrably copper chloride, copper sulfate, copper nitrate, copper acetate, copper tartrate, copper (II) acetylacetonate or copper (II) ETDA.