| 摘要 |
PURPOSE:To obtain a new enzyme useful as a therapeutic agent for herniated disk free from side effect because of absence of impurities, high purity, high specific activity and excellent stability, also useful as a reagent for research having high reproducibility. CONSTITUTION:Purified chondroitinase ABC not containing endotoxin, having contents of nucleic acid and protease of a detection limit. Molecular weight: 100,000 Dalton. Isoelectric point: pH 8.2-pH 8.5. Optimum pH and optimum temperature: optimum pH 8-8.2 and optimum temperature 37 deg.C in the case of chondroitin sulfate as a substrate in a tris-hydrochloric buffer solution. Influence by a metal; inhibition of activity with Zn<2+>, Ni<2+>, etc. End amino acids: Ala of N end and Pro of C end. Specific activity, >=300V/mg, etc. The enzyme is obtained by removing nucleic acid from an extracted solution of the enzyme yielded from a cell producing the enzyme and successively chromatographing the extracted solution with a weak cation exchange resin and a strong cation exchange resin. |
