METHODS FOR PRODUCING HUMAN LYMPHOCYTES AND HUMAN MONOCLONAL ANTIBODIES, AND HUMAN MONOCLONAL ANTIBODIES PRODUCED THEREBY

摘要

A method for producing a human lymphocyte producing an antibody specific to a desired antigen, a method for producing a monoclonal antibody, as well as monoclonal antibodies produced by the method are described. The present invention provides a method for producing a human lymphocyte producing an antibody comprising, in the order mentioned, the steps of: transplanting human lymphocytes to an immunodeficient animal so that the human lymphocytes take in the animal body; immunizing the animal with a desired antigen so as to generate human lymphocytes producing an antibody specific to the antigen; and recovering the human lymphocytes producing the antibody from the animal. The present invention also provides a method for producing a monoclonal antibody comprising, in the order mentioned, the steps of: immortalizing the human lymphocytes producing the antibody, which were obtained by the above-mentioned method of the present invention; cloning the obtained immortalized human-originated lymphocytes producing the antibody; and recovering a monoclonal antibody specific to the desired antigen from the cloned immortalized human-originated lymphocytes.