| 摘要 |
<p>PURPOSE:To provide a novel compound useful for the chemical synthesis of oligonucleotides, facilitating protecting groupremoving reactions, exhibiting proper stability also during basic treatments performed in the synthetic reaction operations, and capable of being readily purified. CONSTITUTION:A compound of the formula (R<1> is H, trityl, alkoxytrityl, n- butyryl; R<2> is H; R<3> is H, OH), e.g. 5'-0-(4,4'-dimethoxytrityl)-N<4>-(n-butyryl)-2'- deoxycytidine. The compound of the formula is produced e.g. by protecting the 3',5'-hydroxyl groups of 2'-deoxycytidine with trimethylsilyl chloride, reacting the protected product with n-butyl chloride, selectively removing the protecting groups of the 3',5'-hydroxyl groups with ammonia water, reacting the obtained intermediate product with 4,4'-dimethoxytrityl chloride without purifying the intermediate product, and finally removing the n-butyryl group from the produced protected nucleotide having the n-butyryl group as the protecting group of the amino group on the outside of the ring.</p> |
