| 摘要 |
PCT No. PCT/EP91/01089 Sec. 371 Date Jan. 29, 1992 Sec. 102(e) Date Jan. 29, 1992 PCT Filed Jun. 6, 1991 PCT Pub. No. WO91/18997 PCT Pub. Date Dec. 12, 1991.For the preparation of cholesterol esterases with variable substrate specificity by cloning of a cholesterol esterase gene, the active center of which has the sequence -Gly-His-Ser-X-Gly-, wherein X signifies an amino acid, into a vector, transformation of a micro-organism with this vector and expression of the cholesterol esterase gene, one exchanges the amino acid X of the active center for another amino acid by mutagenesis.
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