| 摘要 |
The invention generally relates to the discovery of a 23 kD protein, designated herein as Queb, that has specific binding affinity for the purine base Queuine. The invention particularly relates to polypeptides having specific binding reactivity with Queuine and methods of using such polypeptides to purify Queuine and Queuine-containing substances. The invention also relates to antibodies having specific reactivity with Queuine or Queb. Methods for determining the presence and concentration of Queuine and Queb are also provided as well as methods for the diagnosis of a pathological disease or prognosis of a patient having a disease associated with Queuine, Queuine-containing substances such as Queuine-tRNA, or Queb. Kits useful for performing the methods of the present invention are also provided.
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