发明名称 Mass propagation through shoot primordia and regeneration of plants from protoplasts of shoot primordia
摘要 A process for the mass propagation of a woody plant comprising the steps of removing a shoot tip from the woody plant, transplanting the shoot tip to an artificial medium containing inorganic salts and plant growth hormones as main ingredients, and rotary-culturing the shoot tip under illumination to form shoot primordia, and stationary-culturing the shoot primordia in a liquid medium to regenerate shoots. Further, a process for the regeneration of plants comprising the steps of preparing a shoot primordium by rotary-culturing a shoot tip of a plant, treating the shoot primordium with at least one enzyme to prepare a protoplast, culturing the protoplast in an artificial medium containing plant hormones and inorganic and organic salts as main ingredients to form a callus, and incubating the callus in a regeneration medium to regenerate a shoot; plus intermediate materials of the process, and processes for production of the intermediate materials.
申请公布号 US5310673(A) 申请公布日期 1994.05.10
申请号 US19920917677 申请日期 1992.07.22
申请人 OJI PAPER COMPANY, LTD. 发明人 SHIBATA, MASARU;ITO, KAZUYA;DOI, KEIGO;ITO, MASAKI
分类号 A01H4/00;C12N5/02;C12N5/04;(IPC1-7):C12N5/02;C12N5/00 主分类号 A01H4/00
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