| 摘要 |
PROCESS FOR RECOVERING REFRACTILE BODES CONTAINING HETEROLOGOUS PROTEINS FROM MICROBIAL HOSTS A refractile material containing a heterologous protein is recovered from a host microorganism cell culture transformed to produce the protein. One recoveryprocess involves first reducing the ionic strength of the culture medium prior to disruption to a level effective in preventing reaggregation of cellular debris with refractile material after disruption, disrupting the desalted culture, optionally adding material to the disruptate to create a density or viscosity gradient and separating the refractile material from the cellular debris by high-speed centrifugation. Preferably the salt removal step is carried out by diafiltration and the heterologous protein comprises recombinant M-CSF, IL-2 or IFN-.beta..
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