Purification of Mullerian inhibiting substance

摘要

The present invention discloses a method of purifying Mullerian Inhibiting Substance. The method takes advantage of immunoaffinity chromatography to achieve MIS at up to 95% purity. The method provides for a purified MIS product in which contaminating enzymes having MIS proteolytic activity or inhibitors of MIS antiproliferative activity are substantially removed by addition of an alkali metal halide solution or an alkaline earth metal halide solution prior to elution with an acid solution having a pH of between about 2.5 and 4.0.