Site specific double stranded DNA endonuclease

摘要

An endonuclease which cleaves double stranded DNA at a specific site, producing staggered ends with 4 base pair, 3' overhangs, has been isolated, purified and characterized. A method of producing sufficient quantities of the endonuclease for purification and characterization is disclosed. A method to use the endonuclease to cleave DNA, producing fragments useful for gene mapping is also disclosed.