| 摘要 |
A rapid, simple method of directly transfecting a large number of eukaryotic, prokaryotic or plant cells, which retains substantial cell viability is achieved by the present invention. The method includes the steps of contacting with cells adhered to a support, an amount of polynucleotide molecule targeted for transfection into the cells and an amount of particles. A gentle agitation of the cells, polynucleotide molecules and particles permits direct transfection of the polynucleotide molecules into the cells. |
