| 摘要 |
<p>PURPOSE:To simply and quickly detect underwater toxic substance with a microorganism by providing a flow cell having an immobilized microorganism film immobilizing nitrous acid production bacteria. CONSTITUTION:An immobilization microorganism film 1 immobilizing purely cultivated nitrous acid production bacteria is attached to a flow cell 3 set in a constant temperature bath 2, a pump 4 is driven, a valve 5 is opened, first buffer solution 6 containing no microorganism substrate on either surface of the microorganism film 1 is allowed to flow and carried to a detection part 10 having an optical system from the outlet of the flow cell 3. Next, after it is washed with pure water, second buffer solution containing ammonia nitrogen with specified concentration is allowed to flow from the flow cell 3 through the same route as the buffer solution 6 to the detection part 30. Ammoniacal nitrogen is converted into nitrous nitrogen in the flow cell 3 with nitrous acid production bacteria of the microorganism film 1, the concentration of nitrous nitrogen increases in the buffer solution 22, a degree of ultraviolet absorbance becomes high, and since it becomes constant concentration corresponding to the concentration of feed ammonia, the degree of the ultraviolet absorbance becomes constant in the detection part 10.</p> |
