Cathepsin L-specific monoclonal antibodies, hybridomas for producing the same and a method of using the same.

摘要

A monoclonal antibody is disclosed which is reactive specifically with human procathepsin L or with any of the following peptides corresponding to parts of the primary amino acid sequence of human procathepsin L: a peptide comprising the 94th to 107th amino acid sequence: Gly-Phe-Gln-Asn-Arg-Lys-Pro-Arg-Lys-Gly-Lys-Val-Phe-Gln; a peptide comprising the 112th to 125th amino acid sequence: Tyr-Glu-Ala-Pro-Arg-Ser-Val-Asp-Trp-Arg-Glu-Lys-Gly-Tyr; a peptide comprising the 282nd to 295th amino acid sequence: Gly-Tyr-Gly-Phe-Glu-Ser-Thr-Glu-Ser-Asp-Asn-Asn-Lys-Tyr; a peptide comprising the 297th to 310th amino acid sequence: Leu-Val-Lys-Asn-Ser-Trp-Gly-Glu-Glu-Trp-Gly-Met-Gly-Gly; and a peptide comprising the 308th to 321st amino acid sequence: Met-Gly-Gly-Tyr-Val-Lys-Met-Ala-Lys-Asp-Arg-Arg-Asn-His. Also disclosed are a hybridoma characterized by producing the monoclonal antibody and a method of detecting human cathepsin L characterized by employing the monoclonal antibody to detect cathepsin L in a specimen from a human. It is possible to specifically detect human procathepsin L by utilizing a monoclonal antibody according to the present invention. Accordingly, the antibody may be utilized for the diagnosis and therapy of diseases related with human procathepsin L. <IMAGE>