| 摘要 |
An enzyme, exo- alpha -1,4-glucan lyase, capable of successively cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan is disclosed. The enzyme is e.g. selected from the group consisting of alpha -1,4-glucan lyase isolated from an alga and functional derivatives and analogs derived from a nucleotide sequence related to an alga alpha -1,4-glucan lyase. Further, a method of enzymatically cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan, and the degradation product 1,5-anhydrofructose for use as an oxygen radical scavenger or anti-oxidant and/or as a sugar substitute, are disclosed. Additionally, a method of producing an enzyme capable of successively cleaving the terminal alpha -1,4-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan; an antibody which binds to the amino acid sequence of the enzyme, and a DNA or RNA probe which recognizes a nucleotide sequence coding for the enzyme, are described. |
