发明名称 |
Electrode for electrical cell fusion and electrical introduction of nucleic acids. |
摘要 |
<p>This aim of this invention is to make possible efficient, large-scale cell fusion and introduction of nucleic acids by the electrical method. An electrode of which a contact part has an overall cross-section matching the shape of the base of a cylindrical container for cell culture, and wherein electrical cell fusion and electrical introduction of nucleic acids are performed by applying an A.C. voltage to a plurality of conductors so as to generate an electrical field, and applying a D.C. pulse in synchronism, is characterized in that these conductors are disposed such that the cross-section of the contact part consists of a series of concentric rings formed by the conductors, in that the conductors have flat surfaces, and in that the conductors or their surfaces consist of a stable material which does not easily ionize. <IMAGE></p> |
申请公布号 |
EP0594282(A2) |
申请公布日期 |
1994.04.27 |
申请号 |
EP19930305201 |
申请日期 |
1993.07.02 |
申请人 |
DIRECTOR GENERAL OF FRUIT TREE RESEARCH STATION OF JAPAN, MINISTRY OF AGRICULTURE, FORESTRY AND FISHERIES |
发明人 |
OMURA, MITSUO;HIDAKA, TETSUSHI |
分类号 |
C12M1/00;C12M1/42;C12M3/00;C12N15/02;C12N15/09;(IPC1-7):C12M3/00 |
主分类号 |
C12M1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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