METHOD FOR ESTIMATING VIABLE CELL COUNT

摘要

PURPOSE:To obtain a method for estimating the viable cell counts of Bacillus subtilis and a lactic acid bacterium with a high accuracy at a low cost in which the test is sufficiently carried out in a short time. CONSTITUTION:The viable cell counts of Bacillus subtilis and a lactic acid bacterium in Gram-positive bacteria are measured by specifying the bacteria to the Bacillus subtilis and lactic acid bacterium. Since the bacteria are cultured in a specific liquid selective culture medium, microbial species are limited to the Bacillus subtilis and lactic acid bacterium. After culturing the specimen, the culture medium is removed to mix the obtained microbial cell with a physiological saline solution. A fluorescent label AO-10 is then added to the mixture solution and the fluorescent intensity of the mixture solution is subsequently measured. Thereby, the fluorescence derived from the culture medium is eliminated. Because of this, extremely sharp detection of the fluorescence can be carried out simply by culture in a short time. As a result, remarkable reduction in the detection time can be realized.