Gene, vector and transformant for thermostable lipase and preparation of them and thermostable lipase

摘要

A thermostable lipase gene coding for a thermostable lipase, a vector having this gene, a transformant containing this vector, methods for preparing them and for preparing a thermostable lipase by cultivating the transformant are disclosed. The thermostable lipase gene is obtained from a cell belonging to the bacterial strain Pseudomonas sp. KWI-56 by a technique of genetic engineering. The thermostable lipase gene is inserted into a high expression vector, which is introduced into a cell of a host microorganism to prepare a transformant. By cultivating this transformant, a thermostable lipase can be produced.