One step rna and combined one step rna and dna polymerase chain reaction for detection of rare rna or rna and dna

摘要

A one step PCR technique for the detection of rare RNA and a combined one step PCR technique for the simultaneous detection of DNA and RNA is provided which offers several important advantages over traditional PCR. The simplified procedure of the one step RNA PCR and the combined one step PCR technique for the simultaneous detection of DNA and RNA and elimination of the potential for contamination with repeated opening of the reaction tubes reduces both labor intensity and the risk of false-positive results. In addition, the RT-PCR incubation program can be substantially shortened in the one step method and the combined one step PCR technique for the simultaneous detection of DNA and RNA. The increased intensity of reaction products using specimens containing both plus and minus stranded HCV RNA also favors the one step method. Thus, the one step RNA PCR is an attractive alternative to traditional RNA PCR for the detection of HCV RNA. And the combined one step PCR technique for the simultaneous detection of DNA and RNA is an attractive alternative to the separate use of traditional RNA PCR and DNA PCR for the detection of HCV RNA and HBV DNA. The reduced risk of contamination and time required for each assay make these techniques particularly well suited for testing of multiple clinical specimens.