发明名称 |
Controlling cultivation conditions for animal cells |
摘要 |
Stable, long-term cultures of animal cells are accomplished by calculating the ratio of lactic acid formed by the cells to the glucose consumed by the cells (the L/G ratio) and controlling the cultivation conditions to maintain the L/G ratio between the limits of from greater than 0 to 0.6. When the ratio is outside the desired range, the dissolved oxygen concentration in the medium is increased and/or the glucose concentration in the medium is decreased.
|
申请公布号 |
US5304483(A) |
申请公布日期 |
1994.04.19 |
申请号 |
US19900493369 |
申请日期 |
1990.03.14 |
申请人 |
HITACHI, LTD. |
发明人 |
TAKEUCHI, KOUJI;KAWAGUCHI, HIDEO;ISHIBASHI, TADASHI;SHIMIZU, NORIO |
分类号 |
C12M1/36;C12M3/02;(IPC1-7):C12N5/00;C12N5/02;C12N5/06 |
主分类号 |
C12M1/36 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|