| 摘要 |
PCT No. PCT/EP91/02269 Sec. 371 Date Jul. 17, 1992 Sec. 102(e) Date Jul. 17, 1992 PCT Filed Dec. 20, 1990 PCT Pub. No. WO91/10677 PCT Pub. Date Jul. 25, 1991.A method for the concentration and purification of biomolecules is described and comprises introducing the biomolecule into an aqueous/organic solvent system and bringing about a phase separation by addition of salts, where the pH at which the concentration takes place, the solvents suitable for the aqueous/organic solvent system, and the nature of the salt are determined in a series of tests one embodiment concerns concentration of hirudin by using propanol, butanol or acetone a the solvent and sodium chloride as the salt.
|
