| 摘要 |
A method and apparatus which allow accurate spectrophotometric determinations of the concentrations of various hemoglobin species in whole blood without hemolysis or dilution. To overcome complex optical properties of whole blood, the invention employs 1) an optical apparatus (10, 11, 12, 13 and 14), designed to maximize the true optical absorbance of whole blood and to minimize the effects of light scattering on the spectrophotometric measurements of concentrations of various constituent components, and 2) methods to correct the hemoglobin concentration measurements for light scattering and for the effects of the finite bandwidth of the substantially monochromatic light. In the optical apparatus, (10-14), (including an optical cuvette (11)) all optical parameters, such as sample thickness, detector size and shape, sample to detector distance, wavelengths, monochromicity, maximum angle of light capture by detector, are optimal values to minimize the contribution of light scattering to the total optical attenuation of unaltered whole blood and maximize contribution of true optical absorbance. |
