A NOVEL beta 1->6 N-ACETYLGLUCOSAMINYLTRANSFERASE, ITS ACCEPTOR MOLECULE, LEUKOSIALIN, AND A METHOD FOR CLONING PROTEINS HAVING ENZYMATIC ACTIVITY

摘要

The present invention provides a novel (beta)1->6 (N)-acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in (O)-glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 (beta)1->6 (N)-acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed.