Method of controlling the expression of a gene in a cell culture, cell culture vector used in the method and method of making the vector

摘要

To control the production of a protein product by an animal cell culture, a culture of cells is grown to a density greater than one million cells per milliliter wherein the cells have first and second genes under the control of a single promoter with one of said first and second genes being expressed in an early protein which activates said promoter and the other being expressed in a late protein which is said protein product. A starting material for said culture is introduced into it, whereby said culture begins to replicate said early protein for activating said promoter in a self-stimulating manner to cause the other of said first and second genes express itself in said final product. The gene for the early proteins and the gene for the late proteins are artifically positioned into the same genomic region. The first and second promoters are incorporated in the same transactivation region. The vector material is taken from a parvovirus.