Maltooligoside derivative, reagent for determining alpha -amylase activity containing the same as effective ingredient and a process for determining the alpha -amylase activity using same

摘要

The present invention provides a maltooligoside derivative represented by the formula: <IMAGE> wherein n denotes an integer 3-5, R represents an aromatic chromophoric group, X represents a group >CHCH2N3 or >C=CH2, and Y represents a hydrogen atom, a substituted or unsubstituted hydrocarbon group or an alkyl- or arylsulfonyl group, a reagent for determining alpha -amylase activity which comprises said maltooligoside derivative as an effective ingredient, and a process for determining alpha -amylase activity, characterized in that said maltooligoside derivative and coupled enzymes are added to an alpha -amylase containing sample to conduct an enzymatic reaction and a liberated aromatic chromophoric compound is quantitatively determined. The compound of the present invention is very useful as a substrate for determining alpha -amylase activity, and alpha -amylase activity can be easily measured accurately in a short time by using the reagent without affection of the other ingredients contained in the sample.