发明名称 |
Positive selection vector for the bacteriophage P1 cloning system |
摘要 |
Positive selection cassettes are disclosed which contain a lethal gene, a promoter, a repressor sequence overlapping the promoter, and a cloning site between the promoter and the lethal gene. Insertion of a foreign nucleic acid sequence into the cloning site prevents expression of the lethal gene. Expression of the lethal gene under nonrepressed conditions kills a host organism containing a positive selection cassette which does not contain the foreign nucleic acid sequence.
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申请公布号 |
US5300431(A) |
申请公布日期 |
1994.04.05 |
申请号 |
US19910662224 |
申请日期 |
1991.02.26 |
申请人 |
E. I. DU PONT DE NEMOURS AND COMPANY |
发明人 |
PIERCE, JAMES C.;STERNBERG, NAT L. |
分类号 |
C12N1/21;C12N15/64;C12N15/67;C12N15/70;(IPC1-7):C12N15/00;C12N15/11 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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