Method for quantitative measurement of trace enzyme and substrate used therein

摘要

A synthetic substrate used for quantitative analysis of a trace enzyme. The substrate has a molecular structure (B)-(A)-(C) comprising at least one structure (A) catalytically affected by the enzyme to be analysed, at least one photographically active labelling structure (B) linked to the structure (A) and at least one specific adsorbing structure (C) linked to the structure (A). The substrate is used in a quantitative analysis of a trace enzyme, in which the structure (A) or the linkage between the structure (A) and the structure (C), or the linkage between the structure (B) and the structure (A) is cleaved by the action of the analyte enzyme. The reaction product of the enzymatic reaction is separated by allowing the same to contact with an adsorbent for the structure (C). The separated reaction product is developed photographically and then the optical density of the resultant developed silver and/or colored dye is measured.